Dr J. William Allwood Homepage

		Dr J. William (Will) Allwood

Dr J. William Allwood
PDRA Mass Spectrometry

based metabolic profiling,
School of Chemistry,
Manchester Interdisciplinary Biocentre,
The University of Manchester,
131 Princess Street,
Manchester,

M1 7DN

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Personal | Education | Publications & Proceedings | Miscellaneous | Contact

Personal

Dr J. William Allwood
School of Chemistry,

Manchester Interdisciplinary Biocentre,

The University of Manchester,

131 Princess Street, Manchester, M1 7DN, UK

Tel: +44 (0)161 306 5138

E-mail: William.Allwood@manchester.ac.uk

Place of birth: Shrewsbury, Shropshire, UK

Date of birth: 1st September, 1979

Education

Bishops Castle Community College

Ten GCSE’s and three A levels in Chemistry, Biology and Geography

University of Wales, Aberystwyth

BSc (Hons) Microbiology Class 2(i), with First class dissertation (87%) on the rapid characterisation of urinary tract infection bacteria using MALDI-TOF/MS and multivariate analysis.

PhD Metabolomics / Plant pathology; thesis title 'Plant-pathogen interactions are a biochemically rich, novel and challenging target for metabolomic approaches'. This was under the guidance and supervision of Dr Luis A.J. Mur and Prof. Roy Goodacre. We developed a novel dual-metabolomics system based upon the biochemical analysis of both pathogen and host during the interaction of Arabidopsis thaliana with various strains of Pseudomonas syringae pv. tomato. We also generated metabolomic profiles from; Brachypodium distachyon plants when challenged by pathogenic and resistance eliciting strains of Magnaporthe grisea (rice blast disease), and from genetically modified A. thaliana plants expressing individual bacterial avirulence genes under the control of chemical induced gene switches.

Publications

Wedge, D.C., Allwood, J.W., Dunn, W.B., Vaughan, A.A., Simpson, K., Brown, M., Priest, L., Blackhall, F.H., Whetton, A.D., Dive, C., Goodacre, R. (2011). Is serum or plasma more appropriate for inter-subject comparisons in metabolomic studies? An assessment in patients with small-cell lung cancer. Anal. Chem. In Press.

Lloyd, A.J., Allwood, J.W., Winder, C.L., Dunn, W.B., Heald, J.K., Cristescu, S.M., Sivakumaran, A., Harren, J.F.M., Mulema, J., Denby, K., Goodacre, R., Smith, A.R., Mur, L.A.J. (2011). Metabolomic approaches reveal that cell wall modifications play a major role in ethylene-mediated resistance against Botrytis cinerea. Plant J. (On Line First - http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2011.04639.x/full)

Allwood, J.W., De Vos, R.C.H., Moing, A., Deborde, C., Erban, A., Kopka, J., Goodacre, R., Hall, R.D. (2011). Plant metabolomics and its potential for systems biology research: background concepts, technology and methodology. In Methods In Enzymology - Systems Biology. Ed.s N. Hayes & H. Westerhoff. Elsevier. Amsterdam NL. (In Press)

Allwood, J.W., Parker, D., Beckmann, M., Draper, J., Goodacre, R. (2011). FT-ICR-MS for plant metabolite profiling and metabolite identification. In Methods in Molecular Biology: Plant Metabolomics. Ed.s R.D. Hall & N. Hardy. Humana Press Inc. NJ US (In Press)

Kopka, J., Walther, D., Allwood, J.W., Goodacre, R. (2011). Progress in Chemometrics and Biostatistics for Plant Applications, or: A Good Red Wine is a Bad White Wine. In Biology of Plant Metabolomics. Ed. R.D. Hall. Blackwell, Oxford UK (Now Published).

Moing, A., Aharoni, A., Biais, B., Rogachev, I., Meir, S., Brodsky, L., Allwood, J.W., Erban, A., Dunn, W.B., Lorraine Kay, L., de Koning, S., de Vos, C.H.R., Jonker, H., Deborde, C., Maucourt, M., Bernillon, S., Gibson, Y., Hansen, T.H., Husted, S., Goodacre, R., Kopka, J., Schjoerring, J.K., Rolin, D. & Hall, R. (2011) Spatial and temporal metabolic profiling using multiple analytical platforms highlights the crosstalk between primary and secondary metabolites and mineral elements in melon fruit. New Phytology 190: 683-696 (Full Text).

Lowe, R.G.T., Allwood, J.W., Galster, A.M., Urban, M., Daudi, A., Canning, G., Ward, J.L., Beale, M.H., Hammond-Kosack, K.E. (2010). A Combined ¹H Nuclear Magnetic Resonance and Electrospray Ionization-Mass Spectrometry Analysis to Understand the Basal Metabolism of Plant-Pathogenic Fusarium spp. Molecular Plant-Microbe Interactions 23: 1605-1618 (Full Text).

Allwood, J.W., Clarke, A., Goodacre, R. & Mur, L.A.J. (2010) Dual metabolomics: A novel approach to understanding plant-pathogen interactions. Phytochemistry 71: 590-597 (Full Text).

Kim, D.-H., Jarvis, R.M., Allwood, J.W., Batman, G., Moore, R.E., Marsden-Edwards, E., Hampson, L., Hampson, I.N. & Goodacre, R. (2010) Raman chemical mapping reveals site of action of HIV protease inhibitors in HPV16 E6 expressing cervical carcinoma cells. Analytical and Bioanalytical Chemistry 398: 3051-3061 (Full Text)

Kim, D.-H., Jarvis, R.M., Xu, Y., Oliver, A.W., Allwood, J.W., Hampson, L., Hampson, I.N. & Goodacre, R. (2010) Combining metabolic fingerprinting and footprinting to understand the phenotypic response of HPV16 E6 expressing cervical carcinoma cells exposed to the HIV anti-viral drug lopinavir. Analyst 135: 1235-1244 (Full Text)

Allwood, J.W. & Goodacre, R. (2010). An introduction to Liquid Chromatography Mass Spectrometry (LC-MS) instrumentation applied in plant metabolomic analyses. Phytochemical Analysis 21: 33-47(Full Text).

Biais, B., Beauvoit, B., Allwood, J.W., Deborde, C., Maucort, M., Goodacre, R., Rolin, D., Moing, A. (2010). Metabolic acclimation to hypoxia revealed by metabolite gradients in melon fruit. J. Plant Physiol. 167: 242-245 (Full Text).

Allwood, J.W. & Erban, A., deKoning, S., Dunn, W.B., Luedemann, A., Lommen, A., Kay, L., Löscher, R., Kopka, J., Goodacre, R. (2009). Inter-laboratory reproducibility of fast gas chromatography - electron impact – time of flight mass spectrometry (GC-EI-TOFMS) based plant metabolomics. Metabolomics 5: 479-496 (Full Text).

Biais, B. & Allwood, J.W., Deborde, C., Xu, Y., Maucourt, M., Beauvoit, B., Dunn, W.B., Jacob, D., Goodacre, R., Rolin, D., Moing, A. (2009). ¹H-NMR, GC-EI-TOFMS, and dataset correlation for fruit metabolomics: application to spatial metabolite analysis in melon. Anal. Chem. 81: 2884-2894 (Full Text).

Allwood, J.W., Ellis, D.I., Goodacre, R. (2008). Metabolomic technologies and their application to the study of plants and plant-host interactions. Physiologia Plantarum 132: 117-135 (Full Text).

Jansen, J.J., Allwood, J.W., Marsden-Edwards, E., van der Putten, W.H., Goodacre, R., van Dam, N.M. (2008). Metabolomic analysis of the interaction between plants and herbivores. Metabolomics 5(1): 150-161 (Full Text)

Ellis, D.I., Dunn, W.B., Griffin, J.L., Allwood, J.W., Goodacre, R. (2007). Metabolic fingerprinting as a diagnostic tool. Pharmacogenomics 8(9): 1243 -1266 (Full Text).

Allwood, J.W., Ellis, D.I., Heald, J.K., Goodacre, R., Mur, L.A.J. (2006). Metabolomic approaches reveal that phosphatidic and phosphatidyl glycerol phospholipids are major discriminatory metabolites in responses by Brachypodium distachyon to challenge by Magnaporthe grisea. The Plant Journal 46: 351-368. (Full Text).

Conference Proceedings:

Allwood, J.W., Biais, B., Erban, A., Deborde, C., Xu, Y., Maucourt, M., Beauvoit, B., Dunn, W.B., Jacob, D., META-PHOR Consortium, Kopka, J., Goodacre, R., Rolin, D., Moing, A. Multi-platform metabolomic analysis of aroma and spatial metabolite distributions in Cucumis melo. DANSAK10 - Danish Symposium of Analytical Chemistry (Copenhagen, August, 2010).

Allwood, J.W., Erban, A., de Koning, S., Dunn, W.B., Luedemann, A., Lommen, A., Biais, B., Deborde, C., Xu, Y., Maucourt, M., Beauvoit, B., Jacob, D., Kay, L., Löscher, R., Rolin, D., Moing, A., Kopka, J., Goodacre, R. Inter-laboratory reproducibility of fast gas chromatography – electron impact – time of flight mass spectrometry (GC-EI-TOF/MS) based plant metabolomics and applications in spatial metabolite profiling of Charentais melon fruit. The Sixth International Conference of the Metabolomics Society (Amsterdam, June, 2010).

Allwood, J.W., Kim, D.H., Jarvis, R., Dunn, W.B., Xu, Y., Moore, R., Marsden-Edwards, E., Hampson, L., Hampson, I.N., Goodacre, R. Analysis of C33A cervical cell lines exposed to HIV antivirals with MS based metabolic profiling and Raman spatial mapping. The MPF MetaboMeeting (John Innes Research Centre, 5-8^th June, 2009)

Allwood, J.W., Biais, B., Deborde, C., Xu, Y., Maucourt, M., Beauvoit, B., Dunn, W.B., Jacob, D., Goodacre, R., Rolin, D., Moing, A. Poster presentation: META-PHOR, Spatial melon metabolomics. The MPF MetaboMeeting (John Innes Research Centre, 5-8^th June, 2009). The Fifth International Conference of the Metabolomics Society (Alberta, August, 2009).

Allwood, J.W. An Introduction to UPLC and GC-MS based plant metabolomics. Leiden University Metabolomics Workshop (Leiden University, NL, 20-25^th April, 2009, 2010, 2011).

Allwood, J.W., Brown, D.M., Marsden-Edwards, E., Dunn, W.B, Goodacre, R., Turner, S.R. Metabolomic analysis of Arabidopsis thaliana secondary Cell Wall Mutants – looking towards improved feed-stocks. Synthetic Plant Products for Industry Network (SPPI-NET) First Workshop (Collingwood College, Durham, UK, 13-14th January, 2009). Invited speaker at The fifth international workshop on molecular interactions ‘from omics-to-systems’ (Institute of Physiology, Berlin Free University, DE, 16-18^th September, 2009).

Allwood, J.W., Erban, A., de Koning, S., Dunn, W.B., Luedemann, A., Lommen, A., Kay, L., Löscher, R., Kopka, J., Goodacre, R. Poster Presentation: Inter-laboratory reproducibility of fast gas chromatography – electron impact – time of flight mass spectrometry (GC-EI-TOF/MS) based plant metabolomics. Genomes 2 Systems (Manchester Central Convention Complex, UK, 17-19th March, 2008). BBSRC Metabolomics Grant Holders Workshop (St Davids Hotel & Spa, Cardiff, UK, 12-13th May, 2008). Dechema: Trends In Metabolomics (Dechema, Frankfurt, DE, 9-10th June, 2008). The MPF MetaboMeeting (John Innes Research Centre, 5-8^th June, 2009). The Fifth International Conference of the Metabolomics Society (Alberta, August, 2009).

Allwood, J.W., Dunn, W.B., Goodacre, R. Poster Presentation: META-PHOR Partner 5 Initial Investigations. The Third International Conference of the Metabolomics Society (University of Manchester, UK, 10-14th June, 2007).

Allwood, J.W., Dunn, W.B., Wong, S.C.C., Jarvis, R., Heald, J.K., Gaskell, S.J., Goodacre, R., Mur L.A.J. Dual metabolomic analysis of Arabidopsis thaliana cell suspension cultures challenged by Pseudomonas syringae pv. tomato. Plant Science Wales (University of Wales Swansea, 13-14th December, 2004); All Wales Microbiology Meeting (Gregynog Hall, Newtown, 12-13th March, 2005); The First International Conference of the Metabolomics Society (Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan, 20-23rd June, 2005).

Allwood, J.W., Ellis, D.I., Heald, J.K., Goodacre, R., Mur, L.A.J. Poster presentation: Metabolomic approaches reveal that phosphatidic and phosphatidyl glycerol phospholipids are major discriminatory metabolites in responses by Brachypodium distachyon to challenge by Magnaporthe grisea. British Society of Plant Pathology Presidential Meeting (University of Nottingham, 15-18th December, 2003) & Society for Experimental Biology Annual Main Meeting (Heriot-Watt, Edinburgh, 29th March – 2nd April, 2004).

Miscellaneous

I serve as an advisory member of the editorial board for the journal Metabolomics. I am a member of the Society for Experimental Biology (SEB), the Metabolomics society and the Metabolite Profiling Forum. My research interests largely focus upon the profiling and identification of plant and mammalian derived metabolites using a combination of the many hyphenated mass spectrometry based techniques available to our group (GC-TOF/MS, GCxGC-TOF/MS, UPLC-TOF/MS, UPLC-QTOF/MS, FT-ICR/MS, and UPLC -Thermo Hybrid LTQ Orbitrap XL MS system). My research focuses largely upon methodology development and optimisation for plant metabolomic analysis with interest towards nutraceutical and health beneficial metabolites in foods, although I also have committed research interests in clinical applications of metabolic profiling (combined with proteomics) in the study of Cancer biomarkers with the Patterson Institute for Cancer research and also with other researchers based at Saint Mary’s and Wythenshaw Hospitals in Manchester.

After having been schooled and worked in the picturesque South Shropshire countryside (Bishops Castle) for 20 years, I moved to Aberystwyth (Ceredigion) where I studied for my BSc and PhD. I have since worked as a post-doctoral research associate for the Wheat Pathogenesis team at Rothamsted Research and the Bioanalytical Spectroscopy group at the University of Manchester on an EU funded project, META-PHOR – Metabolomics for Plants, Health and Outreach (Hall, 2007) as well as in clinical metabolic profiling applications with Cancer Research UK.

My interests outside work include: listening to music (live and recorded) and playing my guitars; reading; comedy; mountain biking, climbing and badminton. I have also travelled around many of the European countries as well as Japan and Laos. I enjoy eating out as well as drinking fine malt whiskeys, beers and ales - German beer guide to Manchester.