| Operating System | Windows95/98 Windows 2000 and Windows XP Windows NT 4.0 or higher |
| Hardware requirements | PCs that are able to run Win95/98/Win NT/2000/XP 512MB RAM, 1GB or above recommended Hard disk space 35MB |
| Software requirement | Matlab component run time (MCR) |
| Input images | A stack of time series (Karl Zeiss LSM file; TIF; JPG; BMP; Matlab mat file) Note that input images could have more than three channels |
| Language | English ( UK ) |
| Boundary detection method | Level set (Vese 1998) Snake(Kass 1987, Xu 1998) Thresholding |
| Boundary tracking method | Particle filter based on image colour, edge features (Blake 1998, Isard 1998,Nummiaro 2003) Snake(Kass 1987, Xu 1998) Segmentation based (Jones 2005) |
| Cell tracking method | Combination of the above boundary tracking methods |
| Export format | AVI; Tiff; XML; XLS |
Abraham, V. C., Taylor, D. L., Haskins, J. R. 2004 High content screening applied to large-scale cell biology. Trends Biotechnol 22 , 15-22.
Bailey, S. N., Ali, S. M., Carpenter, A. E., Higgins, C. O., Sabatini, D. M. 2006 Microarrays of lentiviruses for gene function screens in immortalized and primary cells. Nat Methods 3 , 117-22.
Nelson, D. E., Ihekwaba, A. E. C., Elliott, M., Gibney, C. A., Foreman, B. E., Nelson, G., See, V., Horton, C. A., Spiller, D. G., Edwards, S. W., McDowell, H. P., Unitt, J. F., Sullivan, E., Grimley, R., Benson, N., Broomhead, D. S., Kell, D. B. & White, M. R. H. 2004 Oscillations in NF- k B signalling control the dynamics of target gene expression. Science 306 , 704-708.
Blake, A. & Isard, M. 1998 Active contours : the application of techniques from graphics, vision, control theory and statistics to visual tracking of shapes in motion .
Doucet, A., De Freitas, N., Gordon, N. 2001 Sequential Monte Carlo methods in practice . Statistics for engineering and information science. New York ; London: Springer.
Isard, M., Blake, A. 1998 CONDENSATION - Conditional density propagation for visual tracking. Int. J. Comp. Vision 29 , 5-28.
Jones, T. R., Carpenter, A. & Golland, P. 2005 Voronoi-based segmentation of cells on image manifolds. In Computer Vision for Biomedical Image Applications. First International Workshop, CVBIA 2005. Proceedings, 21 Oct. 2005 , pp. 535-43 BN - 3 540 29411 2. Beijing, China: Springer-Verlag.
Kass, M., Witkin, A., Terzopoulos, D. 1987 Snakes: active contour models. International Journal of Computer Vision 1 , 321-31.
Nummiaro, K., Koller-Meier, E., Van Gool, L. 2003 An adaptive color-based particle filter. Image Vis. Comput. 21 , 99-110.
Shen, H., Nelson, G., Kennedy, S., Nelson, D., Johnson, J., Spiller, D., White, M.R.H., Kell, D.B. (2006) Automatic tracking of biological cells and compartments using particle filters and active contours. Chemometrics and Intelligent Laboratory Systems 82, 276-282.
Vese, L. A.,Chan, T. F. 2002 A multiphase level set framework for image segmentation using the Mumford and Shah model. International Journal of Computer Vision 50, 271-93.
Xu, C., Prince, J. L. 1998 Generalized gradient vector flow external forces for active contours. Signal Processing 71 , 131-9.